Laboratory in Cell and Molecular Neurobiology|
Spring 2012 not offered
The goals of the course are to introduce laboratory techniques within a framework of solving research problems. This is to enhance the understanding of neuroscience, as well as to provide laboratory skills. Review questions will be included for the end of each laboratory session, original research papers will be read and discussed, and students will be given the opportunity to design experiments through an independent research project. Both quantitative and qualitative approaches will be used to analyze experimental data obtained by the student so that the student will not only gain experience in specific laboratory techniques, but will also gain a feel for the research process itself by active participation in research. In addition to techniques practiced in the course, additional techniques employed in research will be presented through lecture. Techniques will include plasmid engineering and analysis, cell culture growth and transfection, Western blotting, immunoprecipitations, cryostat sectioning, immunohistochemistry, and confocal fluorescence microscopy.
Interpretation, Quantitative Reasoning
Experimental data will be acquired and interpreted in a quantitative fashion.
||Gen Ed Area Dept:
|Course Format: Laboratory||Grading Mode: Graded|
||Prerequisites: (NS&B213 AND MB&B181 AND BIOL182) OR (NS&B213 AND MB&B181 AND BIOL196) OR (NS&B213 AND MB&B195 AND BIOL182) OR (NS&B213 AND MB&B195 AND BIOL196)
||Fulfills a Major Requirement for: (BIOL)(CIS)(NS&B)
|Examination and Assignments: |
One midterm, one term paper, assignments of literature summaries.
|Additional Requirements and/or Comments: |
An additional mandatory 4th hour will be scheduled on Tuesday afternoon at a time convenient to enrolled students. The time in the laboratory and outside readings are estimated to take approximately 8-10 hours/week. Because of the project-oriented nature of the course, students will be expected to return to the lab at various times during the week to complete experiments.
Class discussion and review questions will be devoted to designing experiments and formulating testable hypotheses. Grades are based on laboratory notebooks (with review questions), a midterm written exam and lab practicum, and a term paper based on the independent research project.
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